Sperm movement variables including total motile sperm (TM), rapid sperm (RAP), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), lateral head displacement (ALH) and beat-cross frequency (BCF) after sperm activation was recorded and analyzed using the computer assisted semen analyze system. Sperm samples were diluted and activated by fresh seawater, and subsequently incubated at 26 ☌ for 4 h. This study characterizes spermatozoa movement of the noble scallop, Chlamys nobilis, during the post-activation phase. Sperm motility in the post-activation phase is important for conducting and assessing species-specific artificial fertilization protocols.
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